H23LNBCXX_l02n01_bir_0120_pd_21.35100000045dd6.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H23LNBCXX_l02n01_bir_0120_pd_21.35100000045dd6.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	38358
Sequences flagged as poor quality	0
Sequence length	51
%GC	42

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC	5556	14.48459252307211	No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGT	1402	3.6550393659731997	No Hit
TTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTCT	302	0.7873194639970802	RNA PCR Primer, Index 27 (95% over 21bp)
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACA	150	0.3910527139058345	No Hit
ATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTCTTC	123	0.3206632254027843	TruSeq Adapter, Index 27 (95% over 22bp)
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC	119	0.31023515303196203	No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTT	98	0.2554877730851452	No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA	83	0.21638250169456175	No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA	70	0.18249126648938943	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCG	66	0.1720631941185672	No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCG	65	0.16945617602586163	No Hit
TATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTCTT	55	0.14338599509880598	RNA PCR Primer, Index 27 (100% over 21bp)
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA	53	0.13817195891339487	No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGC	50	0.13035090463527815	No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCA	49	0.1277438865425726	No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACCTGTCTCT	43	0.11210177798633922	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCGTCT	110	0.0	38.863636	45
TGCCGTC	755	0.0	38.74172	45
ACCGGGG	30	1.1194778E-4	37.500004	34
GAACCGG	30	1.1194778E-4	37.500004	32
CGAACCG	30	1.1194778E-4	37.500004	31
CCGGGGG	30	1.1194778E-4	37.500004	35
CCTATCT	925	0.0	32.351353	34
TCCTATC	915	0.0	32.213116	33
CTAGATC	915	0.0	32.213116	28
AGATCCT	915	0.0	32.213116	30
AACCGGG	35	2.7701847E-4	32.142857	33
CGGGGGA	35	2.7701847E-4	32.142857	36
ATCTCGT	925	0.0	32.10811	37
TATCTCG	925	0.0	32.10811	36
TCGTATG	925	0.0	32.10811	40
CTCGTAT	925	0.0	32.10811	39
CTATCTC	925	0.0	32.10811	35
ACTAGAT	905	0.0	32.071823	27
GATCCTA	920	0.0	32.03804	31
TAGATCC	920	0.0	32.03804	29