FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0120_pd_2.35100000045b88.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0120_pd_2.35100000045b88.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82761
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC979911.840117929942847RNA PCR Primer, Index 27 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGT8831.0669276591631325No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4380.5292347844999457No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA4300.5195683957419557No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4020.48573603508899116No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA2780.33590700934014817No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2540.3069078430661785No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2500.3020746486871836No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA2470.29844975290293735No Hit
TTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTCT2430.2936165585239424RNA PCR Primer, Index 38 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCG2140.25857589927622915No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1940.23440992738125443No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA1920.23199333019175697No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1860.2247435386232646No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA1840.22232694143376713No Hit
ATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTCTTC1820.21991034424426967RNA PCR Primer, Index 27 (95% over 24bp)
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1420.17157840045432027No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1340.1619120116963304No Hit
GGTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA1310.1582871159120842No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC1260.1522456229383405No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1250.1510373243435918No Hit
GTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1220.14741242855934558No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA1070.12928794963811457No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCC1000.12082985947487342No Hit
GTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA840.10149708195889369No Hit
ATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA840.10149708195889369No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC11550.040.9090945
ATGCCGT12650.037.35177644
GAGACGA12950.036.83397724
CCCACGA13000.036.69230719
AGACGAA13000.036.69230725
CGAATTG13000.036.69230728
ACGAATT13000.036.69230727
TATCTCG12650.036.64031636
TGCTATC12650.036.64031633
TATGCCG12900.036.62790743
CACGAGA13050.036.55172721
CGAGACG13050.036.55172723
GACGAAT13050.036.55172726
CCACGAG13050.036.55172720
ATTGCTA12750.036.5294131
TCGTATG13000.036.5192340
GTATGCC12950.036.48648542
AGCCCAC13150.036.44486617
ATCTCGT12850.036.42023537
GAATTGC13100.036.41221229
Produced by FastQC (version 0.11.2)