FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0120_pd_19.35100000045d93.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0120_pd_19.35100000045d93.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences183079
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC4914526.843603034755485No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGT110906.057494305736868No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCG30461.66376263798688No Hit
TTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTCT4320.23596370965539465RNA PCR Primer, Index 27 (95% over 21bp)
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACA2360.1289061006450767No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA1970.10760382130118692No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT1100.044.9967245
TGCCGTC51000.043.93797745
ATGCCGT61350.036.52545544
TCACAGC250.002103245835.99737529
TATGCCG64700.034.63426643
CGTATGC64950.034.6048741
TCTCGTA65000.034.57824738
CTCGTAT64850.034.5541539
GTATGCC64850.034.5541542
ATCTCGT64950.034.53558737
TATCTCG64850.034.5194636
TCGTATG65050.034.51708640
CTATCTC64900.034.38886635
CCTATCT64900.034.354234
TCCTATC64700.034.2865333
ATCCTAT64850.034.27660832
GATCCTA64850.034.24191331
CACGAGA65800.034.2262321
GCCCACG66100.034.2070418
CCCACGA66000.034.19069319
Produced by FastQC (version 0.11.2)