Basic Statistics
Measure | Value |
---|---|
Filename | H23LNBCXX_l02n01_bir_0120_pd_16.35100000045d43.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 24021 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 51 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC | 4792 | 19.949211106948088 | TruSeq Adapter, Index 12 (95% over 21bp) |
TTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTCT | 374 | 1.556970983722576 | TruSeq Adapter, Index 12 (95% over 22bp) |
TCTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGT | 270 | 1.1240164855751216 | No Hit |
ATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTCTTC | 182 | 0.7576703717580451 | TruSeq Adapter, Index 12 (95% over 24bp) |
TATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTCTT | 75 | 0.31222680154864496 | RNA PCR Primer, Index 12 (95% over 23bp) |
CTCTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCG | 69 | 0.2872486574247533 | No Hit |
CTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTCT | 27 | 0.11240164855751218 | TruSeq Adapter, Index 12 (95% over 22bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TGCCGTC | 680 | 0.0 | 39.375004 | 45 |
ATGCCGT | 690 | 0.0 | 38.804348 | 44 |
TATGCCG | 695 | 0.0 | 38.52518 | 43 |
GTATGCC | 690 | 0.0 | 38.47826 | 42 |
GCAATCT | 710 | 0.0 | 38.34507 | 34 |
CGAGCCC | 710 | 0.0 | 38.34507 | 15 |
CCGAGCC | 710 | 0.0 | 38.34507 | 14 |
TCTCCGA | 710 | 0.0 | 38.34507 | 11 |
CGAGACT | 710 | 0.0 | 38.34507 | 23 |
AGACTAC | 710 | 0.0 | 38.34507 | 25 |
GAGACTA | 710 | 0.0 | 38.34507 | 24 |
CTCCGAG | 710 | 0.0 | 38.34507 | 12 |
AATCTCG | 705 | 0.0 | 38.297874 | 36 |
TGCAATC | 705 | 0.0 | 38.297874 | 33 |
CGTATGC | 705 | 0.0 | 38.297874 | 41 |
TCGTATG | 705 | 0.0 | 38.297874 | 40 |
CTCGTAT | 705 | 0.0 | 38.297874 | 39 |
GACTACT | 705 | 0.0 | 38.297874 | 26 |
TTGCAAT | 700 | 0.0 | 38.250004 | 32 |
ACTTGCA | 700 | 0.0 | 38.250004 | 30 |