FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0120_pd_16.35100000045d43.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0120_pd_16.35100000045d43.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24021
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC479219.949211106948088TruSeq Adapter, Index 12 (95% over 21bp)
TTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTCT3741.556970983722576TruSeq Adapter, Index 12 (95% over 22bp)
TCTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGT2701.1240164855751216No Hit
ATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTCTTC1820.7576703717580451TruSeq Adapter, Index 12 (95% over 24bp)
TATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTCTT750.31222680154864496RNA PCR Primer, Index 12 (95% over 23bp)
CTCTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCG690.2872486574247533No Hit
CTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTCT270.11240164855751218TruSeq Adapter, Index 12 (95% over 22bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC6800.039.37500445
ATGCCGT6900.038.80434844
TATGCCG6950.038.5251843
GTATGCC6900.038.4782642
GCAATCT7100.038.3450734
CGAGCCC7100.038.3450715
CCGAGCC7100.038.3450714
TCTCCGA7100.038.3450711
CGAGACT7100.038.3450723
AGACTAC7100.038.3450725
GAGACTA7100.038.3450724
CTCCGAG7100.038.3450712
AATCTCG7050.038.29787436
TGCAATC7050.038.29787433
CGTATGC7050.038.29787441
TCGTATG7050.038.29787440
CTCGTAT7050.038.29787439
GACTACT7050.038.29787426
TTGCAAT7000.038.25000432
ACTTGCA7000.038.25000430