FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0120_pd_10.35100000045c7a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0120_pd_10.35100000045c7a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89984
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC1140412.673364153627311TruSeq Adapter, Index 12 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGT15711.7458659317211949No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCG3040.33783783783783783No Hit
TTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTCT1950.2167051920341394TruSeq Adapter, Index 12 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTACAG206.9986255E-445.05
TGCCGTC11350.043.41409745
ATGCCGT13200.037.32954444
CGTATGC13700.036.13138641
CACGAGA13850.036.0649821
TCTCGTA13850.036.0649838
ACGGGAT250.002096821836.03
ACTACTC250.002096821836.012
CTCGTAT13700.035.9671539
TATGCCG13700.035.9671543
TCCGAGC13900.035.93525313
AGCCCAC13900.035.93525317
GAGACTA13900.035.93525324
ATCTCGT13850.035.90252737
ACTACTT13850.035.90252727
AATCTCG13850.035.90252736
GCAATCT13850.035.90252734
CAATCTC13850.035.90252735
CCACGAG13950.035.8064520
TGCAATC13950.035.8064533
Produced by FastQC (version 0.11.2)