Basic Statistics
Measure | Value |
---|---|
Filename | H23LNBCXX_l02n01_bir_0113_pc_96.35100000045b38.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 36971 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 53 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTC | 9127 | 24.686916772605557 | No Hit |
ATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTCTTC | 720 | 1.947472343187904 | RNA PCR Primer, Index 34 (95% over 23bp) |
TCTTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGT | 643 | 1.7392009953747531 | RNA PCR Primer, Index 41 (95% over 21bp) |
TTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTCT | 334 | 0.9034107814232776 | RNA PCR Primer, Index 41 (95% over 23bp) |
TATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTCTT | 161 | 0.43547645451840633 | RNA PCR Primer, Index 34 (95% over 22bp) |
CTCTTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCG | 159 | 0.4300668091206622 | No Hit |
CTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTCT | 53 | 0.1433556030402207 | RNA PCR Primer, Index 41 (95% over 23bp) |
ACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTCTTCTG | 40 | 0.10819290795488355 | RNA PCR Primer, Index 34 (96% over 25bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TGCCGTC | 1080 | 0.0 | 39.166664 | 45 |
ATGCCGT | 1165 | 0.0 | 36.309013 | 44 |
GTATGCC | 1165 | 0.0 | 36.11588 | 42 |
TATGCCG | 1175 | 0.0 | 36.0 | 43 |
CGTATGC | 1175 | 0.0 | 36.0 | 41 |
TCTCGTA | 1165 | 0.0 | 35.92275 | 38 |
TCGTATG | 1180 | 0.0 | 35.847458 | 40 |
CTCGTAT | 1175 | 0.0 | 35.80851 | 39 |
CTCCGAG | 1175 | 0.0 | 35.80851 | 12 |
ATCTCGT | 1170 | 0.0 | 35.76923 | 37 |
TCTCCGA | 1185 | 0.0 | 35.696205 | 11 |
GCATCTC | 1180 | 0.0 | 35.65678 | 35 |
ACTCGAC | 1175 | 0.0 | 35.61702 | 27 |
GGCATCT | 1175 | 0.0 | 35.61702 | 34 |
CCACGAG | 1195 | 0.0 | 35.585773 | 20 |
ACGAGAC | 1195 | 0.0 | 35.585773 | 22 |
ATCTCCG | 1190 | 0.0 | 35.54622 | 10 |
CGAGACT | 1190 | 0.0 | 35.54622 | 23 |
AGACTCG | 1190 | 0.0 | 35.54622 | 25 |
GAGACTC | 1190 | 0.0 | 35.54622 | 24 |