FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pc_96.35100000045b38.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pc_96.35100000045b38.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36971
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTC912724.686916772605557No Hit
ATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTCTTC7201.947472343187904RNA PCR Primer, Index 34 (95% over 23bp)
TCTTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGT6431.7392009953747531RNA PCR Primer, Index 41 (95% over 21bp)
TTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTCT3340.9034107814232776RNA PCR Primer, Index 41 (95% over 23bp)
TATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTCTT1610.43547645451840633RNA PCR Primer, Index 34 (95% over 22bp)
CTCTTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCG1590.4300668091206622No Hit
CTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTCT530.1433556030402207RNA PCR Primer, Index 41 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTCTTCTG400.10819290795488355RNA PCR Primer, Index 34 (96% over 25bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC10800.039.16666445
ATGCCGT11650.036.30901344
GTATGCC11650.036.1158842
TATGCCG11750.036.043
CGTATGC11750.036.041
TCTCGTA11650.035.9227538
TCGTATG11800.035.84745840
CTCGTAT11750.035.8085139
CTCCGAG11750.035.8085112
ATCTCGT11700.035.7692337
TCTCCGA11850.035.69620511
GCATCTC11800.035.6567835
ACTCGAC11750.035.6170227
GGCATCT11750.035.6170234
CCACGAG11950.035.58577320
ACGAGAC11950.035.58577322
ATCTCCG11900.035.5462210
CGAGACT11900.035.5462223
AGACTCG11900.035.5462225
GAGACTC11900.035.5462224