FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pc_92.35100000045abf.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pc_92.35100000045abf.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences747089
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTC81891.0961210779438595No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCG11020.14750585271634303No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATTC8190.10962549308047637No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGT7860.10520834867064031RNA PCR Primer, Index 41 (95% over 21bp)
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGGC7490.10025579281718777No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC9200.036.4399541
GTATGCC9150.035.8989742
ATGCCGT9100.035.8513844
CTCGTAT9400.035.6646339
TATGCCG9200.035.46169343
ACGGCAT9400.035.4252732
GCCCACG9550.035.10445418
CATCTCG9550.035.10445436
ACTCGAC9550.035.10445427
TCGTATG9600.034.92161640
CCCACGA9650.034.74067319
GACTCGA9750.034.3843626
CTCGACG9800.033.9793428
GGCATCT9900.033.86338434
ACGAGAC9900.033.63611622
TCTCGTA10050.033.3579638
CGAGACT10050.033.3579623
CACGAGA10150.033.02931221
TCGACGG10200.032.867429
AGACTCG10350.032.82584425
Produced by FastQC (version 0.11.2)