Basic Statistics
Measure | Value |
---|---|
Filename | H23LNBCXX_l02n01_bir_0113_pc_91.35100000045a96.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 30210 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 52 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTC | 8121 | 26.881827209533267 | No Hit |
TCTTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGT | 595 | 1.9695465077788812 | RNA PCR Primer, Index 41 (95% over 21bp) |
ATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTCTTC | 397 | 1.3141343925852367 | RNA PCR Primer, Index 34 (95% over 23bp) |
TTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTCT | 256 | 0.8474015226746111 | RNA PCR Primer, Index 41 (95% over 23bp) |
CTCTTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCG | 151 | 0.49983449189010265 | No Hit |
TATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTCTT | 105 | 0.3475670307845084 | RNA PCR Primer, Index 34 (95% over 22bp) |
CTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTCT | 42 | 0.13902681231380337 | RNA PCR Primer, Index 41 (95% over 23bp) |
TCTTTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCG | 31 | 0.10261502813637868 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TGCCGTC | 930 | 0.0 | 41.129032 | 45 |
ATGCCGT | 1015 | 0.0 | 37.684727 | 44 |
TCGTATG | 1025 | 0.0 | 37.536583 | 40 |
GTATGCC | 1030 | 0.0 | 37.35437 | 42 |
CGTATGC | 1030 | 0.0 | 37.35437 | 41 |
CGAGCCC | 1030 | 0.0 | 37.35437 | 15 |
CTCGTAT | 1030 | 0.0 | 37.35437 | 39 |
TATGCCG | 1025 | 0.0 | 37.31707 | 43 |
CCGAGCC | 1040 | 0.0 | 37.211536 | 14 |
AGCCCAC | 1035 | 0.0 | 37.173912 | 17 |
CATCTCG | 1005 | 0.0 | 37.164177 | 36 |
GCATCTC | 995 | 0.0 | 37.085426 | 35 |
TCCGAGC | 1045 | 0.0 | 37.033493 | 13 |
ATCTCGT | 1015 | 0.0 | 37.019703 | 37 |
GAGCCCA | 1040 | 0.0 | 36.99519 | 16 |
CACGAGA | 1040 | 0.0 | 36.99519 | 21 |
CCCACGA | 1040 | 0.0 | 36.99519 | 19 |
CGAGACT | 1035 | 0.0 | 36.956524 | 23 |
TCTCGTA | 1030 | 0.0 | 36.917477 | 38 |
GGCATCT | 1000 | 0.0 | 36.899998 | 34 |