FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pc_91.35100000045a96.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pc_91.35100000045a96.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30210
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTC812126.881827209533267No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGT5951.9695465077788812RNA PCR Primer, Index 41 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTCTTC3971.3141343925852367RNA PCR Primer, Index 34 (95% over 23bp)
TTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTCT2560.8474015226746111RNA PCR Primer, Index 41 (95% over 23bp)
CTCTTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCG1510.49983449189010265No Hit
TATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTCTT1050.3475670307845084RNA PCR Primer, Index 34 (95% over 22bp)
CTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTCT420.13902681231380337RNA PCR Primer, Index 41 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCG310.10261502813637868No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC9300.041.12903245
ATGCCGT10150.037.68472744
TCGTATG10250.037.53658340
GTATGCC10300.037.3543742
CGTATGC10300.037.3543741
CGAGCCC10300.037.3543715
CTCGTAT10300.037.3543739
TATGCCG10250.037.3170743
CCGAGCC10400.037.21153614
AGCCCAC10350.037.17391217
CATCTCG10050.037.16417736
GCATCTC9950.037.08542635
TCCGAGC10450.037.03349313
ATCTCGT10150.037.01970337
GAGCCCA10400.036.9951916
CACGAGA10400.036.9951921
CCCACGA10400.036.9951919
CGAGACT10350.036.95652423
TCTCGTA10300.036.91747738
GGCATCT10000.036.89999834