FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pc_9.35100000045069.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pc_9.35100000045069.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35205
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTC1034129.373668512995316RNA PCR Primer, Index 45 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGT11493.263740945888368No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCG2910.8265871325095868No Hit
TTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTCT2740.7782985371396108No Hit
ATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTCTTC2010.5709416276097146RNA PCR Primer, Index 45 (95% over 24bp)
TATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTCTT900.2556455048998722RNA PCR Primer, Index 45 (95% over 23bp)
CTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTCT470.13350376366993325No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCG410.11646073000994178No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC11150.042.3606345
GCCCACG12700.038.07612618
CACGAGA12700.038.07612621
CCCACGA12700.038.07612619
ACGTTCA12650.038.0488227
GACGTTC12650.038.0488226
AGACGTT12650.038.0488225
CCGAGCC12650.038.0488214
TCATTAA12550.037.99357231
CATTAAT12500.037.96561432
AGCCCAC12750.037.92680717
CCACGAG12750.037.92680720
ACGAGAC12750.037.92680722
CGTTCAT12700.037.89902528
GTTCATT12700.037.89902529
CGAGCCC12700.037.89902515
CGAGACG12700.037.89902523
TCCGAGC12700.037.89902513
GAGACGT12700.037.89902524
ATGCCGT12500.037.78568344