Basic Statistics
Measure | Value |
---|---|
Filename | H23LNBCXX_l02n01_bir_0113_pc_9.35100000045069.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 35205 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 46 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTC | 10341 | 29.373668512995316 | RNA PCR Primer, Index 45 (95% over 21bp) |
TCTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGT | 1149 | 3.263740945888368 | No Hit |
CTCTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCG | 291 | 0.8265871325095868 | No Hit |
TTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTCT | 274 | 0.7782985371396108 | No Hit |
ATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTCTTC | 201 | 0.5709416276097146 | RNA PCR Primer, Index 45 (95% over 24bp) |
TATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTCTT | 90 | 0.2556455048998722 | RNA PCR Primer, Index 45 (95% over 23bp) |
CTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTCT | 47 | 0.13350376366993325 | No Hit |
TCTTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCG | 41 | 0.11646073000994178 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TGCCGTC | 1115 | 0.0 | 42.36063 | 45 |
GCCCACG | 1270 | 0.0 | 38.076126 | 18 |
CACGAGA | 1270 | 0.0 | 38.076126 | 21 |
CCCACGA | 1270 | 0.0 | 38.076126 | 19 |
ACGTTCA | 1265 | 0.0 | 38.04882 | 27 |
GACGTTC | 1265 | 0.0 | 38.04882 | 26 |
AGACGTT | 1265 | 0.0 | 38.04882 | 25 |
CCGAGCC | 1265 | 0.0 | 38.04882 | 14 |
TCATTAA | 1255 | 0.0 | 37.993572 | 31 |
CATTAAT | 1250 | 0.0 | 37.965614 | 32 |
AGCCCAC | 1275 | 0.0 | 37.926807 | 17 |
CCACGAG | 1275 | 0.0 | 37.926807 | 20 |
ACGAGAC | 1275 | 0.0 | 37.926807 | 22 |
CGTTCAT | 1270 | 0.0 | 37.899025 | 28 |
GTTCATT | 1270 | 0.0 | 37.899025 | 29 |
CGAGCCC | 1270 | 0.0 | 37.899025 | 15 |
CGAGACG | 1270 | 0.0 | 37.899025 | 23 |
TCCGAGC | 1270 | 0.0 | 37.899025 | 13 |
GAGACGT | 1270 | 0.0 | 37.899025 | 24 |
ATGCCGT | 1250 | 0.0 | 37.785683 | 44 |