FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pc_89.35100000045a6f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pc_89.35100000045a6f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20205
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTC718535.56050482553823No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGT5822.880475129918337RNA PCR Primer, Index 41 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCG2291.13338282603316No Hit
TTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTCT1620.8017817371937639RNA PCR Primer, Index 41 (95% over 23bp)
ATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTCTTC390.19302152932442465RNA PCR Primer, Index 34 (95% over 23bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC7900.042.72151645
ATGCCGT8550.039.47368244
CGAGCCC8750.039.08571215
TCCGAGC8750.039.08571213
CTCCGAG8750.039.08571212
GAGCCCA8700.039.05172316
TCTCCGA8800.038.8636411
AGCCCAC8750.038.8285717
GTATGCC8700.038.79310242
CGAGACT8700.038.79310223
CCACGAG8650.038.7572320
CACGAGA8650.038.7572321
CCCACGA8650.038.7572319
ACGAGAC8650.038.7572322
AGACTCG8650.038.7572325
CCGAGCC8850.038.64406614
GGCATCT8750.038.57142634
TATGCCG8750.038.57142643
CGTATGC8750.038.57142641
TCGTATG8750.038.57142640