Basic Statistics
Measure | Value |
---|---|
Filename | H23LNBCXX_l02n01_bir_0113_pc_89.35100000045a6f.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 20205 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 53 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTC | 7185 | 35.56050482553823 | No Hit |
TCTTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGT | 582 | 2.880475129918337 | RNA PCR Primer, Index 41 (95% over 21bp) |
CTCTTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCG | 229 | 1.13338282603316 | No Hit |
TTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTCT | 162 | 0.8017817371937639 | RNA PCR Primer, Index 41 (95% over 23bp) |
ATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTCTTC | 39 | 0.19302152932442465 | RNA PCR Primer, Index 34 (95% over 23bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TGCCGTC | 790 | 0.0 | 42.721516 | 45 |
ATGCCGT | 855 | 0.0 | 39.473682 | 44 |
CGAGCCC | 875 | 0.0 | 39.085712 | 15 |
TCCGAGC | 875 | 0.0 | 39.085712 | 13 |
CTCCGAG | 875 | 0.0 | 39.085712 | 12 |
GAGCCCA | 870 | 0.0 | 39.051723 | 16 |
TCTCCGA | 880 | 0.0 | 38.86364 | 11 |
AGCCCAC | 875 | 0.0 | 38.82857 | 17 |
GTATGCC | 870 | 0.0 | 38.793102 | 42 |
CGAGACT | 870 | 0.0 | 38.793102 | 23 |
CCACGAG | 865 | 0.0 | 38.75723 | 20 |
CACGAGA | 865 | 0.0 | 38.75723 | 21 |
CCCACGA | 865 | 0.0 | 38.75723 | 19 |
ACGAGAC | 865 | 0.0 | 38.75723 | 22 |
AGACTCG | 865 | 0.0 | 38.75723 | 25 |
CCGAGCC | 885 | 0.0 | 38.644066 | 14 |
GGCATCT | 875 | 0.0 | 38.571426 | 34 |
TATGCCG | 875 | 0.0 | 38.571426 | 43 |
CGTATGC | 875 | 0.0 | 38.571426 | 41 |
TCGTATG | 875 | 0.0 | 38.571426 | 40 |