FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pc_88.35100000045a39.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pc_88.35100000045a39.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55359
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGTC26004.696616629635651No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGT3300.5961090337614481No Hit
TGAACCTGATAATGGAATTGATACGGCTTATTATTCTGGACTTAGATTTGT3090.5581748225220832No Hit
TTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGTCT2540.45882331689517514No Hit
ATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGTCTTC1350.2438627865387742Illumina PCR Primer Index 1 (95% over 22bp)
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA740.13367293484347623No Hit
TATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGTCTT720.13006015282067956Illumina PCR Primer Index 1 (95% over 21bp)
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGGC680.12283458877508624No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCT650.11741541574089127No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTA610.11018985169529796No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCG560.10115789663830634No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCTGA406.5683707E-945.0361673
CTGATAA451.873559E-839.9959876
TGATACG403.382047E-739.37104819
GATACGG403.382047E-739.37104820
TACGGCT403.382047E-739.37104822
CTTATTA403.382047E-739.37104827
CGGCTTA403.382047E-739.37104824
ATACGGC403.382047E-739.37104821
TTCTGGA403.382047E-739.37104834
TCTGGAC403.382047E-739.37104835
CTGGACT403.382047E-739.37104836
TGCCGTC4400.036.30317345
CGGAACC250.002090894235.99638728
CCGGAAC250.002090894235.99638727
CCTGATA504.741196E-835.9963875
GGAATTG458.5094143E-734.99648714
GATTTGT458.5094143E-734.99648745
ACGGCTT458.5094143E-734.99648723
AATTGAT458.5094143E-734.99648716
TATTCTG458.5094143E-734.99648732
Produced by FastQC (version 0.11.2)