FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pc_87.35100000045a1f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pc_87.35100000045a1f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences339722
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGTC62091.82767086029165No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGT10960.3226167277950795No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGAAC207.0289796E-444.99646828
TGCCGTC7800.038.93924745
ACTATAC356.2134823E-638.591123
CGTATGC9000.033.99733441
ATGCCGT9000.033.99733444
GTATGCC9100.033.376542
TATGCCG9150.033.1941243
CTCGTAT9250.033.07848439
CCCACGA9250.033.07848419
TCGTATG9300.032.90064640
CACGAGA9250.032.8352621
GAGACTA9350.032.72470524
GATCTCG9350.032.72470536
CGAGACT9300.032.6587323
ACTAAGC9250.032.59203327
GACTAAG9350.032.24345826
CGAACGG352.8175206E-432.14033530
CACGTGC352.8175206E-432.14033515
ATCTCGT9550.032.03936837
GCCCACG9550.032.03936818
Produced by FastQC (version 0.11.2)