FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pc_86.351000000459f4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pc_86.351000000459f4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences307896
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGTC221857.20535505495362No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGT28460.9243380881856212No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCT10790.35044300672954504No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTA10520.34167381193649804No Hit
TACTAAGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTATTGATATGCTTA9970.323810637358069No Hit
TTACTAAGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTATTGATATGCTT8150.2646997687530855No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA6540.21240938498713852No Hit
TTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGTCT6140.19941798529373553No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5550.18025567074596616No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5360.17408475589159977No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5200.16888819601423857No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCG5040.16369163613687737No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4650.15102502143580948No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA4540.14745238652012368No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGGC4510.14647803154311845No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCG4300.1396575467040819No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA4030.1308883519110349No Hit
GTCAAAGAGTGCTTGAAATTGTCGGGAGGGAAGCGGATGGGGGCCGGCGAT3740.12146958713331775No Hit
ATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGTCTTC3610.11724738223296179Illumina PCR Primer Index 1 (95% over 22bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3610.11724738223296179No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3520.11432431730194612No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA3370.10945254241692No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3370.10945254241692No Hit
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGGA3280.10652947748590433No Hit
GTTGGGCACCGTAACCCGGCTTCCGGTTCATCCCGCATCGCCAGTTCTGCT3270.10620469249356926No Hit
GTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3260.10587990750123419No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATTC3140.1019824875932133No Hit
CTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCCGGGCACGACGGGGCTCT3080.10003377763920283No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAACA207.025857E-444.99918714
TGACCAA207.025857E-444.99918727
TGCCGTC26150.039.922845
GACCACT356.2341696E-638.57073229
TGACTGC356.2341696E-638.57073244
CGGGGTC301.138102E-437.49932517
ATGCCGT28100.037.1523544
CGTATGC28750.036.39064841
TATGCCG28850.036.342543
ATCTCGT28500.036.31513637
GTATGCC28750.036.3123942
CTCGTAT28700.036.29725339
GATCTCG28300.036.1742636
CACGAGA29050.036.16974321
TCGTATG28900.036.12391740
ACTAAGC28600.036.1094927
GAGACTA28750.036.0776124
TGATCTC28400.036.04688335
TCTCGTA28850.036.03054438
GACTAAG28850.036.03054426
Produced by FastQC (version 0.11.2)