FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pc_85.351000000459da.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pc_85.351000000459da.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences261914
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGTC52492.004092946539704No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGT14360.5482715700573471No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTA3230.12332292279145063No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCT2760.10537810120879373No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC5750.041.0866145
GTACCGC250.002104233335.9996956
ACCGCGA250.002104233335.9996958
ATGCCGT6950.033.66877744
TATGCCG7350.032.14258243
CGTATGC7650.031.17620741
GTATGCC7650.030.88209242
CTCGTAT7800.030.86512439
TCGTATG7750.030.77393240
TCTCGTA8050.030.7450838
AGCATGA7650.030.58797631
GATCTCG7650.030.58797636
CGAGACT7800.030.57666423
ACGAGAC7850.030.3819122
AAGCATG7850.030.09528730
CCACGAG7950.029.99974420
CCCATGC300.00514030329.99974432
TACCGCG300.00514030329.9997447
ATCTCGT8000.029.81224637
CTAAGCA8000.029.81224628
Produced by FastQC (version 0.11.2)