FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pc_84.351000000459b0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pc_84.351000000459b0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences421111
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGTC73621.7482326512487205No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGT13730.32604230238583176No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTA6990.16598948970698937No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCT6850.16266495057122707No Hit
TACTAAGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTATTGATATGCTTA4890.11612140267055479No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC8750.041.14122845
CCGTTAC552.7248461E-936.8385961
ATGCCGT9850.036.54677644
CGTATGC10350.035.21599641
TATGCCG10300.034.95007343
GTATGCC10400.034.61401442
GACTAAG10600.034.3854326
CCCACGA10700.034.2743419
GATCTCG10650.034.22436
CGAGACT10750.034.1149323
ACTAAGC10700.034.0640727
CTCGTAT10750.033.90563639
CTAAGCA10750.033.90563628
AGCATGA10950.033.90276731
GCATGAT11000.033.5441332
ATCTCGT10900.033.43904537
ACGAGAC11050.033.39234522
TCGTATG10950.033.28635440
CACGAGA11100.033.2419321
TCTCGTA11100.033.03923438
Produced by FastQC (version 0.11.2)