FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pc_83.35100000045997.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pc_83.35100000045997.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences614950
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGTC66491.0812261159443857No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGT8810.14326367997398162No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCG7880.12814049922757947No Hit
CTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCCGGGCACGACGGGGCTCT6360.10342304252378243No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG8000.037.96614540
ATGCCGT8000.037.96614544
CGTATGC8050.037.73032841
TATGCCG8050.037.73032843
GTATGCC8200.037.04014242
GACTAAG8250.036.81565526
CTCGTAT8350.036.6441939
GCCCACG8350.036.3747518
ACTAAGC8300.036.32280727
CGAGACT8500.035.99752823
GAGACTA8600.035.31734524
CCCACGA8800.035.02600519
TGCCGTC8950.034.69035345
AGCATGA8700.034.65279431
TCTCGTA8900.034.3796638
GATCTCG8850.034.06545636
GCATGAT8850.033.81123732
ACGAGAC9250.033.07880822
CTAAGCA9200.032.76948528
CACGAGA9350.032.484421