FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pc_81.35100000045960.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pc_81.35100000045960.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16612
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGTC832350.10233566096798No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGT7404.454611124488322No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCG3281.9744762822056345No Hit
TTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGTCT1080.6501324343847821No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGCATGATTCGTATGCCGTCT240.14447387430772937No Hit
CTTATACACATCTCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGTCT220.13243438478208525No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGCATGATCGTATGCCGTCTT200.12039489525644113No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCTGTC190.11437515049361907No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCG180.10835540573079702No Hit
CTTATACACACTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGTCT180.10835540573079702No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGCATTATCTCGTATGCCGTC170.10233566096797496No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC9350.043.0748745
GCCGTCT403.192181E-739.37500445
ATGCCGT10600.037.99528544
ATCTCCG11050.036.855210
ACATCTC11050.036.85528
TCTCCGA11100.036.6891911
GAGCCCA11050.036.6515816
CGAGCCC11050.036.6515815
CCGAGCC11050.036.6515814
CACATCT11150.036.5246667
AGCCCAC11100.036.48648517
CCACGAG11100.036.48648520
TCCGAGC11100.036.48648513
CTCCGAG11100.036.48648512
CTCGTAT11050.036.44796439
TCTCGTA11000.036.40909238
CATCTCC11200.036.361619
CATGATC10900.036.33027333
ATGATCT10900.036.33027334
ACTAAGC11150.036.32287227
Produced by FastQC (version 0.11.2)