FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pc_80.35100000045947.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pc_80.35100000045947.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences391187
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCGTC88132.2528867268084065No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCGT10600.2709701498260423No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCG7250.18533335719234023No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGGC5770.14749978910342113No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATTC5690.14545473136888498No Hit
CTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCCGGGCACGACGGGGCTCT5280.13497381047938709No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTA4720.12065840633763393No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4630.11835771638628073No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCT4490.11477886535084243No Hit
GTCAAAGAGTGCTTGAAATTGTCGGGAGGGAAGCGGATGGGGGCCGGCGAT4440.11350070426675735No Hit
GTGCCCAACTGCGCGCTAACCTAGAACCCACAAAGGGTGTTGGTCGATTAA4020.10276415116044245No Hit
GCCTAAGCCCGGGCTTTTGATACGCTTGTGGAGACGTCGCTGCCGTGATCG4000.10225288672680842No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC10500.036.4254745
TATGCCG11000.034.56523543
GTATGCC11100.034.25383842
ATGCCGT11100.034.25383844
CGTATGC11250.033.99710541
CATCTCG11200.033.94799836
TGGACCA11300.033.6518831
TCGTATG11400.033.5497740
CTCGTAT11450.033.40326339
CTCTGGA11550.033.11829428
CACGAGA11700.032.8860221
GACTCTG11650.032.83401526
CCCACGA11700.032.69370319
ACGAGAC11700.032.69370322
ATCTCGT11700.032.6895237
AGACTCT11750.032.5545825
TCTGGAC11750.032.5545829
CCATCTC11700.032.3090735
GTACGGC708.076313E-1032.1524471
CTTATAG352.8141815E-432.148343
Produced by FastQC (version 0.11.2)