FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pc_8.35100000045040.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pc_8.35100000045040.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47339
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGTC719815.205221910053021No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGT4851.0245252328946535RNA PCR Primer, Index 44 (95% over 21bp)
TTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGTCT3900.8238450326369379RNA PCR Primer, Index 44 (95% over 23bp)
ATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGTCTTC3080.6506263334671202RNA PCR Primer, Index 37 (95% over 23bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1700.35911193730328056No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1350.28517712668201695No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCG1340.28306470352140944No Hit
GTACGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA1120.23659139398804369No Hit
TATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGTCTT1110.23447897082743613RNA PCR Primer, Index 37 (95% over 22bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT770.16265658336678004No Hit
CCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCG640.1351950822788821No Hit
CCACTGCTTCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCC630.13308265911827458No Hit
GGTATCAACGCAGAGTACGGGAAGCAGTGGCTGTCTCTTATACACATCTCC600.12674538963645196No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGCTGTCTCTTATACACATCTCCG580.1225205433152369No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC8450.039.13649445
ATGCCGT9000.036.74482344
CTCGTAT9100.036.5882539
CGTATGC9200.036.4350841
TCGTATG9200.036.4350840
TCTCGTA9150.036.38831338
ACCCTAG9150.036.38831327
GACCCTA9150.036.38831326
AGACCCT9150.036.38831325
CCTAGAA9150.036.38831329
GAGACCC9150.036.38831324
TAGAATA9150.036.38831331
TATCTCG9100.036.34103436
GAATATC9100.036.34103433
CCCTAGA9100.036.34103428
AATATCT9050.036.2932334
CACGAGA9250.036.23813621
ACGAGAC9200.036.1905522
GTATGCC9150.036.1424542
TATGCCG9150.036.1424543
Produced by FastQC (version 0.11.2)