FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pc_79.3510000004592d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pc_79.3510000004592d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences296475
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCGTC43471.466228181128257No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCGT10650.35922084492790285No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTA5200.17539421536385869No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCT4740.15987857323551735No Hit
TACTAAGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTATTGATATGCTTA3650.1231132473227085No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC5450.038.80457745
GCCACTC356.235663E-638.56868438
GGCCTCG250.002105328535.99743731
GTATGCC6050.034.9561942
TATGCCG6100.034.66966243
CGTATGC6200.034.11047441
ATGCCGT6200.034.11047444
CCACTCG401.557437E-533.74759739
TCTGGAC6300.033.5690429
GCCCACG6350.032.95596718
CATCTCG6450.032.7883636
TCGTATG6450.032.7883640
CCCACGA6400.032.69849819
CTCTGGA6500.032.53614428
CACGAGA6450.032.44502321
CGAGACT6500.032.19544623
ATCTCGT6650.032.14056837
CTGGACC6850.031.85904530
ACGAGAC6650.031.80761122
GGACCAT6650.031.80224832
Produced by FastQC (version 0.11.2)