FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pc_78.35100000045903.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pc_78.35100000045903.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences353590
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCGTC45141.2766198139087643No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTA8940.2528352046155152No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCGT7620.21550383212194912No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCT7470.21126163070222573No Hit
TACTAAGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTATTGATATGCTTA6850.19372719816736897No Hit
CCGTTACTAAGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTATTGATATG5720.16176928080545266No Hit
TTACTAAGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTATTGATATGCTT4610.13037699029949942No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGGCG207.022411E-445.0055165
TGCCGTC5700.040.65712745
TCCGCCC301.1384502E-437.49929420
GGCCGCT301.1384502E-437.49929414
ATGCCGT6250.036.71930744
CACGAGA6500.036.69161621
CCCACGA6550.036.4115319
CGTATGC6400.036.21025541
TATGCCG6350.036.1410543
ACAACGC250.002103873836.0044143
ATTCGGC250.00210533535.9993232
GCCCACG6650.035.86398718
TCTCGTA6550.035.72451838
CTCGTAT6600.035.45387639
TCGTATG6550.035.38101240
CATCTCG6650.035.18730536
ACGAGAC6800.035.0728722
GTATGCC6550.035.03750642
CTGGACC6750.034.9993430
CGAGACT6850.034.8168623