FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pc_77.351000000458e8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pc_77.351000000458e8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences121347
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCGTC14081.1603088663090146No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATAC2500.20602075040998127No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCCTGTCTCTTATAC1730.14256635928370706No Hit
GATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCG1440.11866795223614923No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCGT1310.10795487321483019No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACTGTCTCTTATACA1260.10383445820663058No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCG1230.1013622092017108No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTAC207.0086494E-444.99958813
TGCCGTC1750.038.57107545
GCCGTCT250.002099797435.9996745
CCCACGA1950.035.768919
CGTATGC1900.035.5259941
ATGCCGT1900.035.5259944
GACTCTG2050.035.12162826
TATGCCG1950.034.61506743
GTATGCC1950.034.61506742
CCACGAG2050.034.0240820
AGACTCT2150.033.48806425
CTGGACC1950.033.4612330
ACGAGAC2100.033.2139822
TCGTATG2050.032.9265340
TCTGGAC2000.032.624729
CACGAGA2150.032.44156321
CATCTCG2100.032.14256336
TGGACCA2000.031.4997131
TCTCGTA2150.031.39506138
GACCATC1950.031.1535633