FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pc_75.351000000458a5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pc_75.351000000458a5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences412087
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCGTC130563.1682630124221345No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCGT22760.5523105557806968No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTA6250.15166700235629854No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCG5180.12570161155290022No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCT5100.12376027392273961No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC13650.041.373445
ATGCCGT15950.035.40733344
TATGCCG16550.034.12368443
GTATGCC16650.033.91873642
CGTATGC16800.033.6158941
CATCTCG16750.033.5819136
CTGGACC16850.033.11554730
ACGAGAC17150.033.06104722
CGAGACT16950.033.05291723
CCCACGA17150.032.9298519
GCCCACG17200.032.83412618
TCGTATG17250.032.73895340
TCTGGAC17100.032.63140529
CTCGTAT17350.032.55025539
CACGAGA17350.032.55025521
TCTCGTA17350.032.55025538
GGACCAT17150.032.53626632
ACTCTGG17150.032.4050727
ATCTCGT17450.032.3637237
CTCTGGA17250.032.21721628