FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pc_74.3510000004588b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pc_74.3510000004588b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences846624
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCGTC62760.7412971992289376No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCG10730.12673867029519598No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG8400.036.15933643
ATGCCGT8550.035.52496344
GTATGCC8600.035.31842442
CGTATGC8850.034.57495541
TCGTATG8850.034.57495540
CATCTCG9000.033.7487236
GCCCACG9350.033.6885618
CCCACGA9300.033.62775419
CTCGTAT9050.033.5622639
CGAGACT9450.032.85589223
CACGAGA9550.032.51185221
TCTGGAC10200.030.88329
ACGAGAC10400.030.28730822
TGGACCA10250.030.07380531
ATCTCGT10150.029.92496937
CTCCGAG10550.029.43015912
GACTCTG10650.028.9442726
TGCCGTC10500.028.92747345
CTGGACC11200.028.52738630
CTCTGGA10950.028.35676228
Produced by FastQC (version 0.11.2)