FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pc_73.35100000045861.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pc_73.35100000045861.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43648
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCGTC1257828.81689882697947No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCGT12512.866110703812317No Hit
TTATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCGTCT3560.815615835777126No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCG3250.7445931085043989No Hit
ATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCGTCTTC2590.593383431085044Illumina PCR Primer Index 7 (95% over 22bp)
TATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCGTCTT1150.2634714076246334Illumina PCR Primer Index 7 (95% over 21bp)
ATTGTAGCTTTTCTCCTCTTAAAAAACAACCTAAGAAACCAAAATTCCTCT1020.2336876832844575No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCG440.10080645161290322No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGCTT253.807273E-545.0436254
CTCCTCT253.8332335E-544.9919713
CAACCTA253.8332335E-544.9919727
CCTAAGA253.8332335E-544.9919730
CTAAGAA253.8332335E-544.9919731
AACCTAA253.8332335E-544.9919728
TCTCCTC253.8332335E-544.9919712
ACCTAAG253.8332335E-544.9919729
TGCCGTC13400.042.30588545
ATGCCGT14900.038.046944
AGCCCAC15250.037.6162417
CTCCGAG15250.037.6162412
GAGCCCA15200.037.59197616
AGCTTTT301.1156064E-437.5363586
CACGAGA15300.037.4933121
CCCACGA15300.037.4933119
CCTCTTA301.1231889E-437.4933115
TTCTCCT301.1231889E-437.4933111
AAGAAAC301.1231889E-437.4933133
CCGAGCC15300.037.4933114
Produced by FastQC (version 0.11.2)