FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pc_72.35100000045848.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pc_72.35100000045848.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences176592
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGCTATGATCTCGTATGCCGTC40662.3024825586663042No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCGCTATGATCTCGTATGCCGT13370.7571124399746307No Hit
CCGTTACTAAGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTATTGATATG3080.17441333695750658No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCT2480.1404367128748754No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCGCTATGATCTCGTATGCCG2180.12344840083355983No Hit
CCTTAATCCTGTCCATTACTCCTTGATTCTCTTCTTCCACTGTTTTCACAT2110.11948446135725288No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTA2090.11835190722116516No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC5850.039.2307745
CCTAGAC250.002102291436.00000430
GGATACC250.002102291436.00000425
CGGTATT250.002102291436.00000444
TTAGATT401.5531254E-533.754
ACAATAG551.11853296E-732.7272723
CGCTATG7200.032.50000430
GATCTCG7200.032.50000436
ATGCCGT7050.032.23404344
GTACATA352.8107804E-432.1428576
ACTCGCT7350.032.14285727
ACCCACT352.8107804E-432.1428578
TATGCCG7150.031.78321643
GTATGCC7300.031.74657642
TCGTATG7300.031.74657640
CGTATGC7400.031.62162241
CTCGTAT7350.031.53061339
TCGCTAT7500.031.529
CTCGCTA7500.031.528
GCTATGA7450.031.40939531
Produced by FastQC (version 0.11.2)