FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pc_71.3510000004582e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pc_71.3510000004582e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences237604
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGCTATGATCTCGTATGCCGTC232019.764566253093381No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCGCTATGATCTCGTATGCCGT40781.716301072372519No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCGCTATGATCTCGTATGCCG11070.46590124745374656No Hit
CCGTTACTAAGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTATTGATATG2890.12163094897392299No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTA2760.11615966061177421No Hit
AACAAGTACCGCGAGGTAAAGATGAAAAGGACTTTGAAAAGAGAGTCAAAG2510.10563795222302653No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA2390.10058753219642767No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTAAC207.025055E-444.99600219
TGCCGTC23800.043.67259245
GGTCTAT301.1321646E-437.528261
ATGCCGT27800.037.4696944
GCCGTTA504.8279617E-836.0195432
TAGTCCG250.002098155236.0195435
GTCCGAC250.00210467135.9968037
TATGCCG29050.035.93484543
CGCTATG29350.035.87415330
GTATGCC29250.035.84296842
GATCTCG29100.035.79578836
ACTCGCT29450.035.75234227
CGAGACT29600.035.7231823
AGACTCG29500.035.69174625
CTCGCTA29500.035.69174628
CGTATGC29450.035.6759541
CTCGTAT29400.035.660139
TCGTATG29400.035.660140
TCGCTAT29550.035.6313529
GACTCGC29550.035.6313526