FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pc_69.351000000457e9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pc_69.351000000457e9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences319927
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGCTATGATCTCGTATGCCGTC97253.0397559443247992No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCGCTATGATCTCGTATGCCGT26410.8255008173739635No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCGCTATGATCTCGTATGCCG4150.1297170917115467No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC10300.042.81449545
ATGCCGT12850.034.31823344
GACTCGC13600.032.75655726
GTATGCC13550.032.54533442
CGAGACT13700.032.51745623
CCCACGA13800.032.44486219
TATGCCG13600.032.42568243
CCACGAG13850.032.32773220
CTCGCTA13700.032.18928
ACTCGCT13700.032.18927
AGACTCG13850.032.16528325
GCCCACG13850.032.16528318
CGCTATG13650.032.1420830
CGTATGC13750.032.07194541
ACGAGAC13900.032.0495822
TCGCTAT13850.032.0028329
CACGAGA14000.031.82065621
CTCGTAT13950.031.61213339
TCGTATG13950.031.61213340
GATCTCG14050.031.38713836
Produced by FastQC (version 0.11.2)