FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pc_68.351000000457b2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pc_68.351000000457b2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences391803
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGCTATGATCTCGTATGCCGTC155723.9744463416563938No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCGCTATGATCTCGTATGCCGT24530.6260799432367797No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCGCTATGATCTCGTATGCCG8780.22409220960533738No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAA207.0237595E-445.004981
TGCCGTC16250.044.03001845
ATGCCGT19100.037.46009444
TATGCCG19750.036.2272343
ACTCGCT19850.036.15807327
CGTATGC19900.036.06722641
CTCGCTA19900.036.06722628
CGCTATG19850.036.04472730
GTATGCC19950.035.8640542
TCGCTAT20050.035.6851829
GACTCGC20350.035.38023826
CTCGTAT20300.035.24570539
TCGTATG20300.035.24570540
GATCTCG20350.034.93798436
GCTATGA20500.034.7920931
CCCACGA20850.034.7476119
AGACTCG20750.034.69820825
TCTCGTA20750.034.69820838
CGAGACT20750.034.69820823
CACGAGA21050.034.52435321
Produced by FastQC (version 0.11.2)