FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pc_66.3510000004578c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pc_66.3510000004578c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences202034
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGCTATGATCTCGTATGCCGTC34621.7135729629666294No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCT8700.4306205886137977No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTA8510.4212162309314274No Hit
TACTAAGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTATTGATATGCTTA7460.3692447805814863No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCGCTATGATCTCGTATGCCGT5220.2583723531682786No Hit
TTACTAAGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTATTGATATGCTT4500.22273478721403328No Hit
GTACGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA2270.11235732599463456No Hit
CCTTAATCCTGTCCATTACTCCTTGATTCTCTTCTTCCACTGTTTTCACAT2120.10493283308750012No Hit
CCGTTACTAAGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTATTGATATG2060.10196303592464634No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGACA253.8694074E-545.0195664
TGCCGTC4150.042.286645
ATGCCGT4600.037.66076744
CCCACGA4700.037.33816519
CGCTATG4650.037.2558130
TATGCCG4700.036.85947443
GTATGCC4700.036.85947442
GACTCGC4650.036.7719726
ACGAGAC4800.036.56028722
CGAGACT4700.036.3807823
CTCGCTA4650.036.28812428
GCCCACG4850.036.18337618
CGTATGC4800.036.09156441
TTAACGT250.002098524736.015654
GACAGTC250.00210363335.997827
GCACACT250.00210363335.9978211
ACTCGCT4750.035.52416627
GATCTCG4900.035.35500336
CACGAGA5000.035.09787421
GCTATGA4950.034.99788331