FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pc_65.35100000045756.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pc_65.35100000045756.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76769
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGCTATGATCTCGTATGCCGTC1484619.33853508577681No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCGCTATGATCTCGTATGCCGT17692.3043155440347016No Hit
TTATACACATCTCCGAGCCCACGAGACTCGCTATGATCTCGTATGCCGTCT4420.5757532337271555No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCGCTATGATCTCGTATGCCG3830.49889929528846283No Hit
ATACACATCTCCGAGCCCACGAGACTCGCTATGATCTCGTATGCCGTCTTC2750.35821750967187277Illumina PCR Primer Index 5 (95% over 21bp)
ATGCACTTGTGTTCCATTTTTCAATTCAATTGTCACAGTTTCGTGACTAAG1350.17585223202073755No Hit
GTTGAAATTGTTTAAGAACCTTTCCTAAGAAAACAAGCAAGCAAGAAAGAA1330.17324701376857848No Hit
TATACACATCTCCGAGCCCACGAGACTCGCTATGATCTCGTATGCCGTCTT1160.15110265862522632No Hit
TGATTGAGTTTCTTTAAATCTGTCCAATCTCAAGTCATTTTTATTTGCCAT1160.15110265862522632No Hit
ATAATTAAGAGATCTGACATTCCGAGATTTACGAATGCTTTCTTCCTGTAG1140.14849744037306725No Hit
AATGGATGTAGTTAAAGGAACCTTTTCTACAGGAGGACAATATCAAATTAT1050.13677395823835142No Hit
TAAGATAACGGTACAATTTGGGTCTTTTACATGTGGCTACCCAGTTTTCTT970.1263530852297151No Hit
TACAGTGGTAGCAAATTACAATTTGTGCATGTCTTGCATATTAGTATAAGG940.12244525785147652No Hit
CTTTTTGAATTTCTCTGATTGTTTTCTTTTGATCATCCTCGTTTTTATCTC910.11853743047323789No Hit
GAATCAATCGAATTGAAAACAATCGAATCGAAAAGAATCGAATCAAAAAAA900.11723482134715836No Hit
ATTCAAAACTATGTCAACTCACAATTCTAAAAACGAAGATTTCTTGCTTTC890.11593221222107881No Hit
CAGTTAGCCAAATTATGAATGCAAATGAAAAGTTCTTGAAGGAAATTAAAA850.11072177571676067No Hit
ACTTCTTAGAAGATACGTGGTATTATCCTCATTGTACAGTTGAGCAAAGTA840.10941916659068113No Hit
TAGTAACACCACTTTTCTACCCTGTTCAAATGTATGTCTACACAGACAAAA830.1081165574646016No Hit
AATGTAATCCATGTTGAAAGAGACAGAAAGGGAAAGAGAGGAAGGAAAGAA820.10681394833852206No Hit
TGTTCTATAGTTTTGAGGGTATAGATCCTTTACCTCTTTGGTTAGGTTTAT800.104208730086363No Hit
AGTGGAAACTAAGTAGCAACTAAATATGAACTGTTACACCAAAACTGTTTA800.104208730086363No Hit
GATTCATAGAAGAGACAATATCCCATCCACTCACTTAGTATGATGTCAACT790.10290612096028344No Hit
ATCTTCAATAAGTTGTGTACTGGAAGTGATTGCTTCATTATTTGTTGTGTT780.10160351183420391No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTATAC253.8439E-545.0254253
AATACTA206.9727143E-445.0254255
TGTCACA253.8587452E-544.99609431
TCCGAGA253.8587452E-544.99609421
TTTCGTG253.8587452E-544.99609439
CCTGTAG253.8587452E-544.99609445
TCGTGAC253.8587452E-544.99609441
TACGAAT253.8587452E-544.99609430
ACAGCCA253.8587452E-544.9960948
CAGGACA253.8587452E-544.99609445
GTGAGGA253.8587452E-544.9960948
CGAGATT253.8587452E-544.99609423
GATCTGA253.8587452E-544.99609411
TACAGCC253.8587452E-544.9960947
AGTACAG253.8587452E-544.99609441
AATAACA253.8587452E-544.99609443
TTGTGGA206.995164E-444.9960940
TAAAGGA206.995164E-444.9960913
TGTGGCA206.995164E-444.9960931
AGGACAA206.995164E-444.9960934
Produced by FastQC (version 0.11.2)