FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pc_64.3510000004573c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pc_64.3510000004573c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences265280
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGTC49891.880654402895054RNA PCR Primer, Index 39 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGT6300.23748492159227982No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCG5960.22466827503015682No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC6200.043.90816545
ATGCCGT6800.040.03391644
CGTTAAT301.1342484E-437.5185431
TATGCCG7300.037.29186643
CTCGTAT7450.036.5410239
CGTATGC7500.036.29741741
GTATGCC7500.036.29741742
CCGCAGT250.00210103636.0110136
GTTATAC7500.035.99743730
TATACAT7600.035.81981732
CATCTCG7600.035.52378536
TCGTATG7750.035.1265340
TGTTATA7750.034.54592529
ATCTCGT8000.034.02882837
TACATCT8000.034.02882834
ATACATC8000.034.02882833
ATTACGT401.5568208E-533.74759716
TCTCGTA8150.033.67858538
ACTGTTA8150.032.8504227
CTGTTAT8600.031.13150628
Produced by FastQC (version 0.11.2)