FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pc_63.35100000045712.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pc_63.35100000045712.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences385468
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGTC76461.9835628379009411RNA PCR Primer, Index 39 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGT10350.26850477860678446No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCG7360.1909367314537134No Hit
GATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCG6230.16162171697780361No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATAC5280.13697635082549006No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACTGTCTCTTATACA5180.13438210175682547No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCG5000.1297124534332292No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCCTGTCTCTTATAC4060.10532651218778212No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCCTGTCTCTTATACA3990.10351053783971692No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC7400.042.26156645
ATGCCGT8200.038.13848544
AGTCGGC301.13882386E-437.49827220
TATGCCG9450.033.09371643
GTATGCC9600.032.57662242
CGTATGC9650.032.4078341
GTCGGCA352.817686E-432.14137621
AAAAAGT5400.032.0943451
TGTTATA9650.031.70838229
CTCGTAT9900.031.58945339
GTTATAC9750.031.38316730
CATCTCG9900.031.3621936
TCGTATG10000.031.2735640
TATACAT9950.031.2045932
CTGTTAT9850.030.83614228
TCTCGTA10300.030.36267738
TACATCT10350.030.21599834
ACTGTTA10100.030.07287227
CGAGACT10250.030.07178323
GCCACGC300.005144224529.99861719