Basic Statistics
Measure | Value |
---|---|
Filename | H23LNBCXX_l02n01_bir_0113_pc_63.35100000045712.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 385468 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 45 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGTC | 7646 | 1.9835628379009411 | RNA PCR Primer, Index 39 (95% over 21bp) |
TCTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGT | 1035 | 0.26850477860678446 | No Hit |
CTCTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCG | 736 | 0.1909367314537134 | No Hit |
GATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCG | 623 | 0.16162171697780361 | No Hit |
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATAC | 528 | 0.13697635082549006 | No Hit |
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACTGTCTCTTATACA | 518 | 0.13438210175682547 | No Hit |
GGTATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCG | 500 | 0.1297124534332292 | No Hit |
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCCTGTCTCTTATAC | 406 | 0.10532651218778212 | No Hit |
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCCTGTCTCTTATACA | 399 | 0.10351053783971692 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TGCCGTC | 740 | 0.0 | 42.261566 | 45 |
ATGCCGT | 820 | 0.0 | 38.138485 | 44 |
AGTCGGC | 30 | 1.13882386E-4 | 37.498272 | 20 |
TATGCCG | 945 | 0.0 | 33.093716 | 43 |
GTATGCC | 960 | 0.0 | 32.576622 | 42 |
CGTATGC | 965 | 0.0 | 32.40783 | 41 |
GTCGGCA | 35 | 2.817686E-4 | 32.141376 | 21 |
AAAAAGT | 540 | 0.0 | 32.094345 | 1 |
TGTTATA | 965 | 0.0 | 31.708382 | 29 |
CTCGTAT | 990 | 0.0 | 31.589453 | 39 |
GTTATAC | 975 | 0.0 | 31.383167 | 30 |
CATCTCG | 990 | 0.0 | 31.36219 | 36 |
TCGTATG | 1000 | 0.0 | 31.27356 | 40 |
TATACAT | 995 | 0.0 | 31.20459 | 32 |
CTGTTAT | 985 | 0.0 | 30.836142 | 28 |
TCTCGTA | 1030 | 0.0 | 30.362677 | 38 |
TACATCT | 1035 | 0.0 | 30.215998 | 34 |
ACTGTTA | 1010 | 0.0 | 30.072872 | 27 |
CGAGACT | 1025 | 0.0 | 30.071783 | 23 |
GCCACGC | 30 | 0.0051442245 | 29.998617 | 19 |