FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pc_62.351000000456f7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pc_62.351000000456f7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences361114
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGTC93032.5761947750571843RNA PCR Primer, Index 39 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGT13340.3694124293159501No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCG7290.2018753080744585No Hit
CTCTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTTACCTCGCGGTACTTG4000.11076834462247379No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC8400.043.12181545
ATGCCGT9600.037.7315944
CGTATGC10200.035.51208541
TATGCCG10200.035.51208543
TCGTATG10250.035.11935840
GTATGCC10400.035.04548642
CTAGGAG458.64542E-735.0168083
CATCTCG10400.034.61282736
GTTATAC10350.034.56266430
TGTTATA10350.034.34528729
CTGTTAT10350.034.34528728
CTCGTAT10550.034.33395839
TATACAT10700.033.8526432
ACTGTTA10500.033.64037327
CGAGACT10650.033.58906623
ACGAGAC10850.033.17727322
TCTCGTA11000.033.13391538
CCACGAG11050.032.78038420
ATCTCGT11000.032.72485437
GCCCACG11100.032.63272518
Produced by FastQC (version 0.11.2)