Basic Statistics
Measure | Value |
---|---|
Filename | H23LNBCXX_l02n01_bir_0113_pc_61.351000000456dd.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 133806 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 49 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGTC | 1990 | 1.4872277775286609 | RNA PCR Primer, Index 39 (95% over 21bp) |
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCG | 277 | 0.2070161278268538 | No Hit |
GTGCCCAACTGCGCGCTAACCTAGAACCCACAAAGGGTGTTGGTCGATTAA | 206 | 0.1539542322466855 | No Hit |
CTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCGGCAGGTGAGTTGTTA | 175 | 0.13078636234548524 | No Hit |
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATTC | 170 | 0.12704960913561425 | No Hit |
TCTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGT | 146 | 0.10911319372823342 | No Hit |
CTCCTTAGCGGATTTCGACTTCCATGACCACCGTCCTGCTGTCTTAATCGA | 139 | 0.103881739234414 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CCCACGA | 220 | 0.0 | 39.88636 | 19 |
GTTATAC | 220 | 0.0 | 39.88636 | 30 |
CTCGTAT | 225 | 0.0 | 39.0 | 39 |
ATGCCGT | 225 | 0.0 | 39.0 | 44 |
TGCCGTC | 225 | 0.0 | 39.0 | 45 |
CGTATGC | 230 | 0.0 | 38.152172 | 41 |
TATGCCG | 230 | 0.0 | 38.152172 | 43 |
TCGTATG | 230 | 0.0 | 38.152172 | 40 |
ATACATC | 230 | 0.0 | 38.152172 | 33 |
TGTTATA | 225 | 0.0 | 38.0 | 29 |
CGAGACT | 225 | 0.0 | 38.0 | 23 |
CCCTCTG | 30 | 1.1345392E-4 | 37.500004 | 9 |
GCCCACG | 235 | 0.0 | 37.340427 | 18 |
TCTCGTA | 235 | 0.0 | 37.340427 | 38 |
TATACAT | 235 | 0.0 | 37.340427 | 32 |
ACTGTTA | 230 | 0.0 | 37.173912 | 27 |
GTATGCC | 240 | 0.0 | 36.562504 | 42 |
ACGAGAC | 250 | 0.0 | 36.000004 | 22 |
AACCGTG | 25 | 0.0021004758 | 36.0 | 7 |
GTCGCGC | 50 | 4.8241418E-8 | 36.0 | 8 |