FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pc_61.351000000456dd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pc_61.351000000456dd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences133806
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGTC19901.4872277775286609RNA PCR Primer, Index 39 (95% over 21bp)
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCG2770.2070161278268538No Hit
GTGCCCAACTGCGCGCTAACCTAGAACCCACAAAGGGTGTTGGTCGATTAA2060.1539542322466855No Hit
CTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCGGCAGGTGAGTTGTTA1750.13078636234548524No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATTC1700.12704960913561425No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGT1460.10911319372823342No Hit
CTCCTTAGCGGATTTCGACTTCCATGACCACCGTCCTGCTGTCTTAATCGA1390.103881739234414No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCACGA2200.039.8863619
GTTATAC2200.039.8863630
CTCGTAT2250.039.039
ATGCCGT2250.039.044
TGCCGTC2250.039.045
CGTATGC2300.038.15217241
TATGCCG2300.038.15217243
TCGTATG2300.038.15217240
ATACATC2300.038.15217233
TGTTATA2250.038.029
CGAGACT2250.038.023
CCCTCTG301.1345392E-437.5000049
GCCCACG2350.037.34042718
TCTCGTA2350.037.34042738
TATACAT2350.037.34042732
ACTGTTA2300.037.17391227
GTATGCC2400.036.56250442
ACGAGAC2500.036.00000422
AACCGTG250.002100475836.07
GTCGCGC504.8241418E-836.08