FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pc_60.351000000456b3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pc_60.351000000456b3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences420679
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGTC155353.6928394333922068RNA PCR Primer, Index 39 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGT25300.6014086750229986No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCG11410.27122818110721003No Hit
CTTTCTAAGAGTCGAGTTGTTTGGGAATGCAGCTCAAAATGGGTGGTAAAT9390.22321057148086781No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCC9080.21584153237979553No Hit
ACTAAGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTATTGATATGCTTAA8080.1920704385053687No Hit
GCTTTGTGATACACTTTCTAAGAGTCGAGTTGTTTGGGAATGCAGCTCAAA7690.18279971189434224No Hit
CCCCAATATTTAGCCTTAGATGGCATTTACCACCCATTTTGAGCTGCATTC7400.17590609467075846No Hit
GTGATACACTTTCTAAGAGTCGAGTTGTTTGGGAATGCAGCTCAAAATGGG6990.16615994618224347No Hit
GCTCAAAATGGGTGGTAAATGCCATCTAAGGCTAAATATTGGGGAGAGACC6810.16188114928484665No Hit
GAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCT6620.15736464144870554No Hit
GTACTTGTTCGCTATCGGTCTCTCCCCAATATTTAGCCTTAGATGGCATTT6160.14642993826646922No Hit
GAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCC6140.14595451638898066No Hit
TCACTGTACTTGTTCGCTATCGGTCTCTCCCCAATATTTAGCCTTAGATGG6070.1442905398177708No Hit
TACTAGGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTATTGATATGCTTA5290.12574908659571787No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCTT5270.12527366471822934No Hit
CTGTACTTGTTCGCTATCGGTCTCTCCCCAATATTTAGCCTTAGATGGCAT5150.12242113345329812No Hit
ATATTTAGCCTTAGATGGCATTTACCACCCATTTTGAGCTGCATTCCCAAA5010.11909318031087836No Hit
CTATCGGTCTCTCCCCAATATTTAGCCTTAGATGGCATTTACCACCCATTT4960.11790462561715703No Hit
AAAAAGTACTCTGCGTTGATACCACCCGTACTCTGCGTTGATACCTGTCTC4900.1164783599846914No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC17050.043.67654445
ATGCCGT19700.037.80127344
CGGTCCG250.002106494535.996869
TATGCCG20950.035.54582643
GTATGCC21150.035.20969442
CGTATGC21400.035.11375841
TGTTATA21500.034.9504429
TCGTATG21550.034.8693540
GTTATAC21550.034.8693530
CATCTCG21500.034.63651336
CTCGTAT21550.034.55615239
CGAGACT21550.034.55615223
TCTCGTA21650.034.50045838
ACGAGAC21650.034.50045822
TATACAT21700.034.42096332
CCACGAG21950.034.1314220
CTGTTAT22000.034.05385228
CACGAGA22050.033.97662721
CCCACGA22000.033.95158819
ATACATC21900.033.90115733
Produced by FastQC (version 0.11.2)