H23LNBCXX_l02n01_bir_0113_pc_6.35100000044ff1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H23LNBCXX_l02n01_bir_0113_pc_6.35100000044ff1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	162671
Sequences flagged as poor quality	0
Sequence length	51
%GC	46

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGTC	7240	4.4507011083721135	No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGT	788	0.48441332505486534	RNA PCR Primer, Index 44 (95% over 21bp)
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	400	0.24589508886033773	No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA	376	0.2311413835287175	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCG	363	0.2231497931407565	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	338	0.2077813500869854	No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	314	0.19302764475536513	No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	290	0.17827393942374486	No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA	265	0.16290549636997373	No Hit
CTCTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTTACCTCGCGGTACTTG	261	0.16044654548137036	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	244	0.14999600420480602	No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	233	0.14323388926114675	No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGGC	225	0.13831598748393997	No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA	221	0.1358570365953366	No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCG	219	0.1346275611510349	No Hit
GTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	191	0.11741490493081128	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	184	0.11311174087575537	No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	177	0.10880857682069944	No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATTC	172	0.10573488820994524	No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA	164	0.10081698643273848	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
TGCCGTC	865	38.7559	45
ATGCCGT	910	36.839397	44
TAGAATA	940	35.663677	31
CTCGTAT	950	35.525105	39
CCCTAGA	945	35.47498	28
GAGACCC	965	35.439205	24
ACCCTAG	960	35.389416	27
TCTCGTA	960	35.389416	38
TATCTCG	955	35.339108	36
CGAGACC	975	35.306488	23
CTAGAAT	950	35.28827	30
GTATGCC	950	35.28827	42
AGAATAT	950	35.28827	32
GAATATC	945	35.236893	33
ATCTCGT	965	35.20605	37
CGTATGC	960	35.15505	41
CACGAGA	980	35.12635	21
TATGCCG	955	35.103516	43
AATATCT	950	35.051434	34
TCGTATG	965	34.9729	40

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content