FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pc_59.3510000004569a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pc_59.3510000004569a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences389011
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGTC106352.7338558549758236RNA PCR Primer, Index 39 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGT30280.7783841588027074No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCG8560.22004519152414712No Hit
CCTTAATCCTGTCCATTACTCCTTGATTCTCTTCTTCCACTGTTTTCACAT3920.10076835873535711No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC10500.042.4226345
GCCGTCT606.4173946E-933.74527745
ATGCCGT13750.032.39546644
TATGCCG14650.030.405343
CGTATGC15000.030.14577941
GCCAGGC300.005143407729.9996627
CATCTCG15000.029.99965736
CGAGACT15150.029.99965723
CGTCGGG300.00514664229.99580214
CTCGTAT15050.029.89614939
GTATGCC15100.029.79715342
CCACGAG15300.029.70554220
GTTATAC15300.029.70554230
ACGAGAC15400.029.51265122
TGTTATA15350.029.46220629
CCCACGA15450.029.41714119
TCGTATG15400.029.36277440
GCCCACG15550.029.22796218
CACGAGA15750.029.14252321
TCTCGTA15450.029.12213938
Produced by FastQC (version 0.11.2)