FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pc_58.35100000045670.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pc_58.35100000045670.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences308084
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGTC47621.5456823463730671RNA PCR Primer, Index 39 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGT16440.5336207008478208No Hit
CCTTAATCCTGTCCATTACTCCTTGATTCTCTTCTTCCACTGTTTTCACAT7470.24246634034873607No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTA4870.1580737720881318No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCT4260.13827397722699003No Hit
GTTTTCACATGATCTCCTTCAACAATCTTCTTCTTTTTCTCCTTCTTCTTC4130.13405434881395983No Hit
GGTGAAGACGGTGAGAAGAAGAAGAAGGAGAAAAAGAAGAAGATTGTTGAA3510.11392996715181573No Hit
GTCCATTACTCCTTGATTCTCTTCTTCCACTGTTTTCACATGATCTCCTTC3430.11133327274379715No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCG3230.10484153672375067No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC5750.041.08577345
GCGGTCG250.002105018435.99896216
GCGCGGC250.002105018435.99896211
CGGTCGC250.002105018435.99896217
GGGCCGG352.8160948E-432.1419341
GGGGCCG352.8160948E-432.1419340
ATGCCGT7450.031.71049544
TATGCCG7650.030.88146243
CATCTCG7650.030.58735336
GTATGCC7650.030.58735342
ACTGTTA7850.030.3812927
GTTATAC7900.030.18900130
GGGCTAG300.00514220429.99913637
GAGTCCG300.00514220429.9991369
CGCTGCA300.00514220429.99913621
GGGACGC300.00514220429.99913618
GACGCAG300.00514220429.99913620
GGCGCGG300.00514220429.99913610
GCCGTCT300.00514220429.99913645
GGATGGG453.4833258E-529.99913435
Produced by FastQC (version 0.11.2)