H23LNBCXX_l02n01_bir_0113_pc_57.35100000045657.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H23LNBCXX_l02n01_bir_0113_pc_57.35100000045657.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	140864
Sequences flagged as poor quality	0
Sequence length	51
%GC	42

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGTC	28309	20.096689004997728	RNA PCR Primer, Index 39 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGT	6444	4.574625170377102	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCG	1792	1.2721490231712858	No Hit
TTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGTCT	868	0.6161971830985915	RNA PCR Primer, Index 39 (100% over 21bp)
ATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGTCTTC	196	0.13914129940935938	RNA PCR Primer, Index 39 (95% over 24bp)
TATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGTCTT	168	0.11926397092230803	RNA PCR Primer, Index 39 (95% over 23bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAACGTA	20	7.014107E-4	44.997517	18
TGCCGTC	2910	0.0	41.28638	45
ATAGCTT	30	1.13521295E-4	37.49793	18
TAAGCCA	30	1.13521295E-4	37.49793	18
TACTAAC	25	0.0021014207	35.998013	7
AGGATAC	25	0.0021014207	35.998013	17
ATGCCGT	3620	0.0	33.126625	44
TCTCGTA	3835	0.0	31.738796	38
CACGAGA	3870	0.0	31.626163	21
CTCGTAT	3835	0.0	31.621462	39
TATGCCG	3800	0.0	31.616674	43
CGAGACT	3865	0.0	31.608862	23
ATCTCGT	3855	0.0	31.574133	37
ACGAGAC	3880	0.0	31.54465	22
CGTATGC	3840	0.0	31.521696	41
CCACGAG	3890	0.0	31.521395	20
CCCACGA	3885	0.0	31.504051	19
CGAGCCC	3875	0.0	31.46923	15
TCGTATG	3840	0.0	31.463104	40
GTATGCC	3820	0.0	31.451141	42