FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pc_56.3510000004563d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pc_56.3510000004563d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences188043
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACCGCTGATCTCGTATGCCGTC40952.1776933999138497No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACCGCTGATCTCGTATGCCGT10900.5796546534569221No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC4900.040.86686345
CGCGGTA250.0021027635.99957741
ATGCCGT6300.031.7853444
CTACCGC6300.031.07106228
TACCGCT6400.030.58557529
TATGCCG6550.030.57215943
ACTACCG6450.030.34847827
GTATGCC6600.030.34055142
CCTCGCG300.00513673929.99964338
ACCTCGC300.00513673929.99964337
CTCGCGG300.00513673929.99964339
CGTATGC6700.029.88770741
ACCGCTG6550.029.88514330
CCCACGA6650.029.77408419
GATCTCG6650.029.77408436
CGAGACT6600.029.65874123
GACTACC6550.029.54163626
GCCCACG6800.029.11730218
CTCGTAT6900.029.02139539
TCGTATG6900.029.02139540
Produced by FastQC (version 0.11.2)