FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pc_55.35100000045613.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pc_55.35100000045613.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences351580
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACCGCTGATCTCGTATGCCGTC3744610.650776494681153No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACCGCTGATCTCGTATGCCGT72842.0717902042209455No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTACCGCTGATCTCGTATGCCG19850.5645941179816827No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCTT5640.16041868138119347No Hit
GGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCA5420.15416121508618238No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCTT4840.13766425849024405No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA4810.13681096763183345No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACTGTCTCTTA4780.13595767677342283No Hit
TATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCTTA4490.12770919847545367No Hit
CCTTAATCCTGTCCATTACTCCTTGATTCTCTTCTTCCACTGTTTTCACAT3920.1114966721656522No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCCTGTCTCTTATACACATCTC3700.10523920587064112No Hit
AAAAAGTACTCTGCGTTGATACCACCCGTACTCTGCGTTGATACCTGTCTC3670.1043859150122305No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC39500.043.6319545
ATGCCGT46600.036.98415844
GCCGTCT1200.035.62421445
TATGCCG48700.035.4817643
ACTACCG49050.035.4120627
GTATGCC48800.035.40905442
CGCTGAT48750.035.39921632
TACCGCT48900.035.38265229
CTACCGC49100.035.37599628
GCTGATC48850.035.3728133
GACTACC49250.035.3139426
CCGCTGA49050.035.2744531
CGAGACT49450.035.2621123
ACCGCTG49150.035.24845530
ATCTCGT49300.035.23248737
GATCTCG49200.035.21263536
AGACTAC49600.035.20083225
ACGAGAC49650.035.16538222
CCACGAG49600.035.1554720
CGTATGC49350.035.151241