FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pc_54.351000000455f8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pc_54.351000000455f8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences223488
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACCGCTGATCTCGTATGCCGTC24281.0864117983963344No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACCGCTGATCTCGTATGCCGT4480.20045819014891178No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCT2330.104256156930126No Hit
CCGTTACTAAGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTATTGATATG2260.10112399770904927No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTAGG253.8622537E-545.036281
TGCCGTC2950.042.7080445
TACCGCT3250.038.7657629
CCCACGA3300.038.17840219
CTACCGC3300.038.17840228
ATGCCGT3300.038.17840244
ACGAGAC3400.037.7172122
CGAGACT3350.037.60857423
ACCGCTG3500.037.28237530
CACGAGA3450.037.17058621
CCACGAG3450.037.17058620
GCCCACG3400.037.05550418
CGTATGC3400.037.05550441
TATGCCG3400.037.05550443
GTATGCC3450.036.5184742
ACTACCG3450.036.5184727
TCGTATG3300.036.1331340
GCGCGCT250.002104409735.99677711
GCTGATC3500.035.99677733
GACTACC3500.035.99677726
Produced by FastQC (version 0.11.2)