FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pc_53.351000000455eb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pc_53.351000000455eb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences412465
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACCGCTGATCTCGTATGCCGTC53411.2948977488999067No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACCGCTGATCTCGTATGCCGT16300.39518504600390336No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC6550.039.1578845
AGCGCCA356.2412055E-638.56904220
GGGGCTA356.2412055E-638.56904236
TCACCCC250.002101177336.0152324
CGCCGGA250.00210618635.9977715
GTGACGT250.00210618635.997779
ATGCCGT7350.034.5896944
TATGCCG7450.034.12540443
GCGCCAT401.5588119E-533.7479121
AAGCGCC401.5588119E-533.7479119
GTATGCC7700.033.01743342
CTACCGC7750.032.8044228
TACCGCT7700.032.72524329
ACTACCG7850.032.67313427
GACTACC8000.032.6229826
GATCTCG8100.031.94246536
CCCACGA8050.031.8613819
ACCGCTG7850.031.81331630
CGTATGC8100.031.66470541
CTCGTAT8100.031.66470539
Produced by FastQC (version 0.11.2)