FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pc_52.351000000455c1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pc_52.351000000455c1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences444831
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACCGCTGATCTCGTATGCCGTC34440.7742266164003858No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACCGCTGATCTCGTATGCCGT6230.14005318873909417No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTA5920.13308424997358548No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCT5610.12611531120807679No Hit
CCGTTACTAAGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTATTGATATG4470.1004876009091093No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC4000.039.93610445
TACCCGG301.1375246E-437.507126
ATGCCGT4200.037.49868844
CTACCGC4400.037.32824328
TACCGCT4450.036.9088229
ACTACCG4650.035.80520227
GTATGCC4500.035.4987642
ACCGCTG4650.035.32134630
TATGCCG4550.035.10866543
CGAGACT4700.034.94558723
CGTATGC4850.034.32869341
CCCACGA4800.034.21755619
CGTCGGG401.5587977E-533.7488214
GACTACC4900.033.51923426
CCGTTAC1500.033.0136871
GCCCACG5000.032.84885418
CTCGTAT5100.032.64591639
ATCTCGT5050.032.52361337
ACGAGAC5050.032.52361322
GATCTCG5150.032.32896836
Produced by FastQC (version 0.11.2)