FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pc_51.351000000455a8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pc_51.351000000455a8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences238838
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACCGCTGATCTCGTATGCCGTC46211.9347842470628627No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACCGCTGATCTCGTATGCCGT6470.27089491621936207No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTA2850.11932774516617958No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCG2570.1076043175709058No Hit
TACTAAGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTATTGATATGCTTA2420.10132390993058055No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCCTG253.88235E-544.99832512
TGCCGTC5600.038.56999245
TCCCCCT301.13746486E-437.49860411
ACGCTGC250.00210415935.9986638
CCCACGA5950.035.92303519
GCCCACG6100.035.40851618
CACGAGA6050.035.3292621
ACCGCTG6000.035.24868430
ATGCCGT6100.035.03967744
TACCGCT6050.034.95737529
TATGCCG6150.034.75480343
TCGTATG6150.034.75480340
ACTACCG6100.034.67083727
GACTACC6250.034.55871626
CGTATGC6200.034.4745241
CTCGTAT6200.034.4745239
GTATGCC6200.034.4745242
ACGAGAC6200.034.4745222
CTACCGC6150.034.3889628
CTCCGAG6400.034.10029212
Produced by FastQC (version 0.11.2)