FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pc_50.3510000004558e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pc_50.3510000004558e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences177660
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACCGCTGATCTCGTATGCCGTC21001.1820330969267139No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTA3280.18462231228188675No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACCGCTGATCTCGTATGCCGT3250.18293369357199146No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCT3030.17055048969942588No Hit
TACTAAGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTATTGATATGCTTA2410.1356523696949229No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC2550.040.5839245
ATGCCGT2750.037.63236244
ACTACCG2950.035.84364727
TATGCCG2900.035.68586343
GCCCACG2950.035.08101318
GACTACC2950.035.08101326
CCCACGA3000.034.4963319
CTACCGC3000.034.4963328
ACGAGAC3000.034.4963322
TACCGCT3000.034.4963329
GTATGCC3050.033.9308242
CGAGACT3050.033.9308223
GCGCCTC401.5543104E-533.74641411
ACAGCGC401.5543104E-533.7464148
ACCGCTG3000.033.7464130
GATCTCG3000.033.7464136
CACGAGA3100.033.38354521
GCTGATC3050.033.1931933
TCGTATG3150.032.8536540
ATCTCGT3200.032.34031337
Produced by FastQC (version 0.11.2)