FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pc_5.35100000044fe4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pc_5.35100000044fe4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences387609
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGTC59701.5402119145840267No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGT9690.24999419518122643RNA PCR Primer, Index 44 (95% over 21bp)

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCCTC207.02938E-444.9975513
TGCCGTC7600.040.8530445
ATGCCGT8550.036.31381244
CTAGGGG250.002101954836.0119744
CGTATGC8800.035.79350741
TATCTCG8850.035.5912836
CCCTAGA8850.035.33705528
TATGCCG8800.035.2821743
GAGACCC8950.035.19361524
GTATGCC8900.034.88574242
ACCCTAG9050.034.80473327
CTCGTAT9100.034.613539
CACGAGA9150.034.42435521
AGACCCT9200.034.23726725
CCCACGA9250.034.05219719
TCGTATG9350.033.68800740
ACGAGAC9350.033.68800722
TAGAATA9250.033.32250631
ATCTCGT9650.033.10700637
GCCCACG9600.032.8107118
Produced by FastQC (version 0.11.2)