FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pc_49.35100000045564.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pc_49.35100000045564.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89555
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACCGCTGATCTCGTATGCCGTC77498.652783205851152No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACCGCTGATCTCGTATGCCGT9821.0965328569035788No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTACCGCTGATCTCGTATGCCG2430.271341633632963No Hit
TTATACACATCTCCGAGCCCACGAGACTACCGCTGATCTCGTATGCCGTCT2140.23895929875495506No Hit
ATTTGATTCCATTTGATGTTGATTCCATTCGATTCCATTTTATGATGATTC1730.19317737703087487No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTCAC302.1432588E-644.99944324
CTACCCG253.862248E-544.9994435
GGCATGG253.862248E-544.99944345
CGACTAG253.862248E-544.99944335
TCACACG206.9988857E-444.9994429
CGTCTTC206.9988857E-444.9994445
GGTCACT206.9988857E-444.9994425
CGAAGAC206.9988857E-444.9994434
TGCCGTC8100.040.2772845
TACCCGA301.13161994E-437.499546
GAATGGC301.13161994E-437.4995441
AAACGAC301.13161994E-437.4995432
AACGACT301.13161994E-437.4995433
ACCCGAA301.13161994E-437.499547
CCCGAAT301.13161994E-437.499548
ATATACG250.00209115336.019671
TGTTTGC250.002096899335.99955434
AGCAACC250.002096899335.99955414
GCAACCA250.002096899335.99955415
TTATGGA250.002096899335.99955443