FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pc_48.3510000004554b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pc_48.3510000004554b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences257281
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCGTC70902.75574177650118RNA PCR Primer, Index 20 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCGT20820.8092319292913196No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCG4430.17218527602115974No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC7400.042.5620545
TACGTGT250.002103406236.0023355
ATGCCGT9400.033.50629444
CCGTTAC551.1193697E-732.7357561
TATGCCG9900.031.81405843
CGTATGC10050.031.78692441
CATCTCG10100.031.63571236
TCGTATG10100.031.62956240
GTATGCC10000.031.4959242
ACCTGTG10200.031.3255627
TGTGGAC10200.030.88435430
CCCACGA10350.030.87156719
TGGACAT10300.030.80296532
GGACATC10300.030.80296533
CCTGTGG10250.030.73369628
GACATCT10250.030.73369634
CTCGTAT10400.030.71717339
CTGTGGA10350.030.43675429
GCCCACG10500.030.43054418
GTGGACA10500.030.21624431
Produced by FastQC (version 0.11.2)